Amino Acid Column Hplc

It is based on ultra high purity and all hole spherical silica gel.

Amino acid column hplc.

However pre column derivatization has gained importance and can be achieved by a broader range of derivatization reagents. Amino acid analysis on eclipse plus c18 2 1 3 0 or 4 6 x 50mm 1 8µ column. Optimized stationary phase for the separation of amino acids and other amine containing compounds. Full chromatographic resolution of isobaric compounds like leu and ile.

Since most of the amino acids have no absorption in the ultraviolet. Description of amino acid hplc columns. The nh2 group is a group where n has a lone e. The use of internal and external standards is crucial.

Hydrolysed samples amino acids are derivatized for sensitive detection separated by hplc. Pre column derivatizing reagents most typically used for amino acid analysis are o phthalaldehyde phenyl isothiocyanate pitc fluorescamine and dansyl chloride. Whether you are looking to separate proteins peptides or amino acids the intrada series of columns has a solution for you. Experimental conditions aaa on production 1200sl using eclipse plus c18 2 1 or 4 6 x 50mm 1 8u.

Nh2 hplc columns description. It uses ultra high purity fully porous spherical silica as a matrix to provide a good peak for amino acid analysis using a unique bonding process and a more thorough tailing process. In an amino column you have an nh2 group at the end of the carbon chain attached to the silica. The nh2 hplc column uses the innovative filler bonding technology unique to hawach.

Luna 5 µm c18 2 100 å lc column 250 x 4 6 mm ea part. Therefore pre column derivatization can be considered appropriate when intending to analyze a somewhat limited variety of samples with high sensitivity. Lc ms analysis of amino acids without derivatization. Post column derivatization was typical.

The characteristics of the column will be affected by the chemical properties of this functional group.